Page 33 - CIBEROBN2016-ENG

Epigenomics-Obesity Platform
The Epigenomics-Obesity Platform is a system shared by the Fundación Ramón Domínguez and the CIBEROBN.
• In the first quarter of 2016 the measures required were taken for putting out the public tender for an array scanner which was published in the State Gazette on 13 February 2016.
• On 8 February 2016 the documents were sent for the application for technical staff associated with the CIBER in the call for Technical Support Staff of the MINECO (State Gazette of 7 December 2015). Financing for this application was not granted (resolution 01/05/2016).
• In April 2016 a call was issued for one place for a predoctoral researcher associated with the CIBER and a predoctoral researcher joined us on 1 June to carry out the epigenetic techniques and perform her doctoral thesis in this area.
• In September 2016 a specific agreement was signed for cooperation between the CIBER and the Fundación Ramón Domínguez for promoting and furthering research in the thematic area of Physiopathology of Obesity and Nutrition. The agreement materialises in joint cooperation by creating the Platform for Epigenomics of Obesity by the joint contribution of media by the Foundation and the CIBER. Under this agreement the Foundation will supply the installations and support resources and the CIBER will provide the ILLUMINA Array HiScan plus the components required for manual processing of the Infinium tests described in the Infinium Option Starter Package 220V, as well as the technical staff required for the management and development of the platform.
• In September 2016 the HiScan equipment for methylation arrays was installed.
• In December 2016 the researchers and technical staff of the Epigenomics of Obesity Platform were given training by ILLUMINA in order to carry out the protocols required for analysis of the samples in the HiScan Equipment.
• The following scientific work has been done since the establishment of the platform:
1. Data has been generated on a piece of research work done in cooperation between the Casanueva and Fernández-Real groups of the CIBEROBN on the methylation pattern in visceral adipose tissue associated with resistance to insulin in patients with morbid obesity. The following publication was a result of this work: CRUJEIRAS AB, DIAZ-LAGARES A, MORENO-NAVARRETE JM, SANDOVAL J, HERVAS D, GOMEZ A, RICART W, CASANUEVA FF, ESTELLER M, FERNÁNDEZ-REAL JM. Genome-wide DNA methylation pattern in visceral adipose tissue differentiates insulin-resistant from insulin-sensitive obese subjects. Transl Res. 2016 Dec;178:13-24.e5.
2. The study of “Evaluation of epigenetic changes in breast cancer associated with obesity” was carried out. The methylome of these samples was analysed with the HiScan equipment, the bioinformatic and statistical analyses were performed and the results were compiled for a publication which is currently being reviewed in the Endocrine-related Cancer journal. The Casanueva and Tinahones group of the CIBEROBN cooperated in this .
3. Different coordination meetings were held between the group of Casanueva and Tinahones of
the CIBEROBN to carry out the study on “Evaluation of the colon cancer epigenome associated with obesity” in the framework of the Complications of Obesity Programme of the CIBEROBN. At present the data on the methylome of the samples in this study were generated with the HiScan equipment, now awaiting the bioinformatic and statistical analysis.
4. Epigenetic analyses have been performed cooperating in a study on cancer which has given rise
to the following publication: DÍAZ-LAGARES A, CRUJEIRAS AB, ET AL. Epigenetic inactivation of the p53- induced long noncoding RNA TP53 target 1 in human cancer. Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):E7535-E7544.
OBN
Platforms 33